I wonder if this is the result of some tool that intercepts all traffic in Layer 2 and rewrites everything to put a MitM proxy while keeping the original IP addresses. It can even be running in a different machine, with the traffic coming through an actual NIC and cable (so the OS being analyzed does not see strange network interfaces nor changes behavior when it detects that it is running in a VM).
The PCAP in the referenced tweet was created using PolarProxy, which decrypts and re-encrypts TLS traffic while saving the decrypted traffic to a PCAP file.
Does somebody know about such a tool?
It does not explain the port 80, however.